expression host origami 2 (de3) cells (Millipore)
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expression host origami 2 (de3) cells
Expression Host Origami 2 (De3) Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+host+origami+2+(de3)+cells/expression+host+origami+2++de3++cells/pmc04157425-90-47-101
Average 90 stars, based on 1 article reviews
Expression Host Origami 2 (De3) Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+host+origami+2+(de3)+cells/expression+host+origami+2++de3++cells/pmc04157425-90-47-101
Average 90 stars, based on 1 article reviews
expression host origami 2 (de3) cells - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome"
Article Title: Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome
Journal: Acta Crystallographica. Section F, Structural Biology Communications
doi: 10.1107/S2053230X14015520
Figure Legend Snippet: Macromolecule cloning and expression conditions
Techniques Used: Clone Assay, Expressing, Plasmid Preparation
Related Articles
Construct:Article Title: Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome Article Snippet: The final construct encoded full-length CBM_E1 fused to an N-terminal His tag with a thrombin protease cleavage site for tag removal. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 DNA source Sugarcane soil metagenome Forward primer † 5′-TATATAT CATATG AGCGCATCATGCGGTAGC-3′ Reverse primer † 5′-ATA GGATCC TTA CCAGTTATCGAACTTCACATT-3′ Cloning vector pJET Expression vector pET-28a Expression host Origami 2 (DE3) cells GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"KJ917170","term_id":"684179993","term_text":"KJ917170"}} KJ917170 Open in a separate window † Restriction sites are shown in bold and the stop codon is underlined. caption a8 Macromolecule cloning and expression conditions Recombinant CBM_E1 was expressed in Clone Assay:Article Title: Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome Article Snippet: The final construct encoded full-length CBM_E1 fused to an N-terminal His tag with a thrombin protease cleavage site for tag removal. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 DNA source Sugarcane soil metagenome Forward primer † 5′-TATATAT CATATG AGCGCATCATGCGGTAGC-3′ Reverse primer † 5′-ATA GGATCC TTA CCAGTTATCGAACTTCACATT-3′ Cloning vector pJET Expression vector pET-28a Expression host Origami 2 (DE3) cells GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"KJ917170","term_id":"684179993","term_text":"KJ917170"}} KJ917170 Open in a separate window † Restriction sites are shown in bold and the stop codon is underlined. caption a8 Macromolecule cloning and expression conditions Recombinant CBM_E1 was expressed in Plasmid Preparation:Article Title: Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome Article Snippet: The final construct encoded full-length CBM_E1 fused to an N-terminal His tag with a thrombin protease cleavage site for tag removal. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 DNA source Sugarcane soil metagenome Forward primer † 5′-TATATAT CATATG AGCGCATCATGCGGTAGC-3′ Reverse primer † 5′-ATA GGATCC TTA CCAGTTATCGAACTTCACATT-3′ Cloning vector pJET Expression vector pET-28a Expression host Origami 2 (DE3) cells GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"KJ917170","term_id":"684179993","term_text":"KJ917170"}} KJ917170 Open in a separate window † Restriction sites are shown in bold and the stop codon is underlined. caption a8 Macromolecule cloning and expression conditions Recombinant CBM_E1 was expressed in Expressing:Article Title: Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome Article Snippet: The final construct encoded full-length CBM_E1 fused to an N-terminal His tag with a thrombin protease cleavage site for tag removal. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 DNA source Sugarcane soil metagenome Forward primer † 5′-TATATAT CATATG AGCGCATCATGCGGTAGC-3′ Reverse primer † 5′-ATA GGATCC TTA CCAGTTATCGAACTTCACATT-3′ Cloning vector pJET Expression vector pET-28a Expression host Origami 2 (DE3) cells GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"KJ917170","term_id":"684179993","term_text":"KJ917170"}} KJ917170 Open in a separate window † Restriction sites are shown in bold and the stop codon is underlined. caption a8 Macromolecule cloning and expression conditions Recombinant CBM_E1 was expressed in Recombinant:Article Title: Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome Article Snippet: The final construct encoded full-length CBM_E1 fused to an N-terminal His tag with a thrombin protease cleavage site for tag removal. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 DNA source Sugarcane soil metagenome Forward primer † 5′-TATATAT CATATG AGCGCATCATGCGGTAGC-3′ Reverse primer † 5′-ATA GGATCC TTA CCAGTTATCGAACTTCACATT-3′ Cloning vector pJET Expression vector pET-28a Expression host Origami 2 (DE3) cells GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"KJ917170","term_id":"684179993","term_text":"KJ917170"}} KJ917170 Open in a separate window † Restriction sites are shown in bold and the stop codon is underlined. caption a8 Macromolecule cloning and expression conditions Recombinant CBM_E1 was expressed in |